Cloned (Comment) | Organism |
---|---|
expressing full-length CaLOX1 in Escherichia coli as a fusion protein with an N-terminal His tag. Overexpression of CaLOX1 in Arabidopsis (Arabidopsis thaliana) conferres enhanced resistance to Pseudomonas syringae pv tomato, Hyaloperonospora arabidopsidis, and Alternaria brassicicola | Capsicum annuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0203 | - |
arachidonate | pH 6.5, 25°C | Capsicum annuum | |
0.114 | - |
linoleate | pH 6.5, 25°C | Capsicum annuum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | Capsicum annuum | - |
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | Capsicum annuum Nockwang | - |
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | D3TTH9 | Nockwang | - |
Capsicum annuum Nockwang | D3TTH9 | Nockwang | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | expression of CaLOX1 is differentially induced in pepper leaves not only during Xanthomonas campestris pv. vesicatoria infection but also after exposure to abiotic elicitors. Transient expression of CaLOX1 in pepper leaves induces the cell death phenotype and defense responses | Capsicum annuum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Capsicum annuum | ? | - |
? | |
arachidonate + O2 | - |
Capsicum annuum Nockwang | ? | - |
? | |
linoleate + O2 | - |
Capsicum annuum | (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | - |
Capsicum annuum | (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | no formation of 13-hydroperoxy-(10E,12Z)-octadecadienoate. The R/S stereoconfiguration of the product is not determined | ? | |
linoleate + O2 | - |
Capsicum annuum Nockwang | (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | - |
Capsicum annuum Nockwang | (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | no formation of 13-hydroperoxy-(10E,12Z)-octadecadienoate. The R/S stereoconfiguration of the product is not determined | ? |
Synonyms | Comment | Organism |
---|---|---|
9-lipoxygenase | - |
Capsicum annuum |
9-LOX | - |
Capsicum annuum |
CaLOX1 | - |
Capsicum annuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Capsicum annuum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
arachidonate | pH 6.5, 25°C | Capsicum annuum | |
8.7 | - |
linoleate | pH 6.5, 25°C | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Capsicum annuum |
Organism | Comment | Expression |
---|---|---|
Capsicum annuum | expression of CaLOX1 is induced 1 h after infection by the virulent strain Ds1, peaks at 20 h, and decreased thereafter. Expression of CaLOX1 is significantly increased by mock inoculation. The induction of CaLOX1 transcript in mock-inoculated or virulent Xcv inoculated leaves may be due, in part, to the wounding effect. Treatment with ethylene, SA, NaCl, and methyl viologen significantly induces CaLOX1 in pepper leaves | up |
General Information | Comment | Organism |
---|---|---|
malfunction | CaLOX1-silenced pepper plants are more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection | Capsicum annuum |
physiological function | CaLOX1 positively regulates defense and cell death responses to microbial pathogens | Capsicum annuum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
arachidonate | pH 6.5, 25°C | Capsicum annuum | |
0.076 | - |
linoleate | pH 6.5, 25°C | Capsicum annuum |